SPLAZERS

NAME

splazers - Split-map read sequences

SYNOPSIS

splazers [OPTIONS] <GENOME FILE> <READS FILE>
splazers [OPTIONS] <GENOME FILE> <READS FILE 1> <READS FILE 2>

DESCRIPTION

SplazerS uses a prefix-suffix mapping strategy to split-map read sequences.If a SAM file of mapped reads is given as input, all unmapped but anchoredreads are split-mapped onto anchoring target regions (specify option -an),if a Fasta/q file of reads is given, reads are split-mapped onto the wholereference sequence.

(c) Copyright 2010 by Anne-Katrin Emde.

REQUIRED ARGUMENTS

ARGUMENT 0 INPUT_FILE

A reference genome file. Valid filetypes are: .sam[.*], .raw[.*], .gbk[.*], .frn[.*], .fq[.*], .fna[.*], .ffn[.*], .fastq[.*], .fasta[.*], .faa[.*], .fa[.*], .embl[.*], and .bam, where * is any of the following extensions: gz, bz2, and bgzf for transparent (de)compression.

READS List of INPUT_FILE’s

Either one (single-end) or two (paired-end) read files. Valid filetypes are: .sam[.*], .raw[.*], .gbk[.*], .frn[.*], .fq[.*], .fna[.*], .ffn[.*], .fastq[.*], .fasta[.*], .faa[.*], .fa[.*], .embl[.*], and .bam, where * is any of the following extensions: gz, bz2, and bgzf for transparent (de)compression.

OPTIONS

-h, --help

Display the help message.

--version

Display version information.

Main Options::

-o, --output OUTPUT_FILE

Change output filename. Default: <READS FILE>.result.

-f, --forward

only compute forward matches

-r, --reverse

only compute reverse complement matches

-i, --percent-identity DOUBLE

Percent identity threshold. In range [50..100]. Default: 92.

-rr, --recognition-rate DOUBLE

set the percent recognition rate In range [80..100]. Default: 99.

-pd, --param-dir STRING

Read user-computed parameter files in the directory <DIR>.

-id, --indels

Allow indels. Default: mismatches only.

-ll, --library-length INTEGER

Paired-end library length. In range [1..inf]. Default: 220.

-le, --library-error INTEGER

Paired-end library length tolerance. In range [0..inf]. Default: 50.

-m, --max-hits INTEGER

Output only <NUM> of the best hits. In range [1..inf]. Default: 100.

--unique

Output only unique best matches (-m 1 -dr 0 -pa).

-tr, --trim-reads INTEGER

Trim reads to given length. Default: off. In range [14..inf].

-mcl, --min-clipped-len INTEGER

min. read length for read clipping In range [1..inf]. Default: 0.

-qih, --quality-in-header

quality string in fasta header

-ou, --outputUnmapped OUTPUT_FILE

output filename for unmapped reads

-v, --verbose

verbose mode

-vv, --vverbose

very verbose mode

Output Format Options::

-a, --alignment

dump the alignment for each match

-pa, --purge-ambiguous

purge reads with more than max-hits best matches

-dr, --distance-range INTEGER

only consider matches with at most NUM more errors compared to the best (default output all)

-of, --output-format INTEGER

Set output format. 0 = RazerS, 1 = Enhanced Fasta, 2 = Eland, 3 = GFF, 4 = SAM. In range [0..4].

-gn, --genome-naming INTEGER

Select how genomes are named. 0 = use Fasta id, 1 = enumerate beginning with 1. In range [0..1]. Default: 0.

-rn, --read-naming INTEGER

Select how reads are named. 0 = use Fasta id, 1 = enumerate beginning with 1. In range [0..1]. Default: 0.

-so, --sort-order INTEGER

Select how matches are sorted. 0 = read number, 1 = genome position. In range [0..1]. Default: 0.

-pf, --position-format INTEGER

Select begin/end position numbering (see Coordinate section below). 0 = gap space, 1 = position space. In range [0..1]. Default: 0.

Split Mapping Options::

-sm, --split-mapping INTEGER

min. match length for prefix/suffix mapping (to disable split mapping, set to 0) Default: 18.

-maxG, --max-gap INTEGER

max. length of middle gap Default: 10000.

-minG, --min-gap INTEGER

min. length of middle gap (for edit distance mapping about 10% of read length is recommended) Default: 0.

-ep, --errors-prefix INTEGER

max. number of errors in prefix match Default: 1.

-es, --errors-suffix INTEGER

max. number of errors in suffix match Default: 1.

-gl, --genome-len INTEGER

genome length in Mb, for computation of expected number of random matches In range [-inf..10000]. Default: 3000.

-an, --anchored

anchored split mapping, only unmapped reads with mapped mates will be considered, requires the reads to be given in SAM format

-pc, --penalty-c INTEGER

percent of read length, used as penalty for split-gap Default: 2.

Filtration Options::

-oc, --overabundance-cut INTEGER

Set k-mer overabundance cut ratio. In range [0..1].

-rl, --repeat-length INTEGER

Skip simple-repeats of length <NUM>. In range [1..inf]. Default: 1000.

-tl, --taboo-length INTEGER

Set taboo length. In range [1..inf]. Default: 1.

-lm, --low-memory

decrease memory usage at the expense of runtime

Verification Options:

-mN, --match-N

N matches all other characters. Default: N matches nothing.

-ed, --error-distr STRING

Write error distribution to FILE.